HISTOPATHOLOGICAL STUDY OF LYMPHOCYSTIS DISEASE (LCD) IN INTENSIVE CULTURED FALSE CLOWNFISH, Amphiprion ocellaris (Cuvier, 1830)

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Bambang Setyo Sihananto
Endang Wijayanti

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Bambang Setyo Sihananto, Balai Perikanan Budidaya Air Tawar Mandiangin

Balai Perikanan Budidaya Air Tawar Mandiangin

Endang Wijayanti, Balai Perikanan Budidaya Laut Batam

Balai Perikanan Budidaya Laut Batam

References

Alexandrowicz, J.S. (1951). Lymphocystis tumours in the Red mullets (Mullus surmuletus L.). Journal of the Marine Biological Vol. 30. No. 2.
Alonso, M.C., Cano, I., Garcia-Rosado, E., Castro, D., Lamas, J., & Barja, J.L. (2005). Isolation of lymphocystis disease virus (LCDV) from Sole (Solease negalensis) and Black Spot Sea Bream (Pagellus bogaraveo). Journal of Fish Disease, 28, 221-228.
Aly, S. M., Mansour, S. M. & Thabet, R. Y. (2018). Pathological and molecular characterization of lymphocystis disease virus (LCDV) in sea bream fish in egypt. Suez Canal Veterinary Medicine Journal SCVMJ. 23:109-126.
Anderson, J.I, & Conroy, D.A. (1968). The significance of disease in preliminary attempts to raise flatfish and salmonids in sea water. Bull Off Int Epizoot. 69(7-8):1129.
Aneer, G & Ljungberg, O. (1976). Lymphocystis disease in Baltic herring (Clupea harengus var. membras L.). Journal of Fish Biology. Vol. 8. 5: 345-350. https://doi.org/10.1111/j.1095-8649.1976.tb03963.x
Armed Forces Institute of Pathology. (1968). Manual of Histologic and Special Staining Technics, 3rd ed. McGraw-tIill Book Company, New York.
American Veterinary Medical Association. (2020). AVMA guidelines for the Euthanasia of animals 2020 Edition.
Bangham, R.V, & Hunter, H.W. (1939). Studies on fish parasites of lake eErie. distribution studies. Zoologica, 24: 383-448.
Benkaroun, J., Bergmann, S. M., Römer-Oberdörfer A., Demircan, M.D, Tamer, C., Kachh, G.R, Weidmann, M. (2022). New Insights into Lymphocystis Disease Virus Genome Diversity. Viruses. 14(12):2741. https://doi.org/10.3390/v14122741.
Borrego, J. J., Valverde, E. J., Labella, A. M., & Castro, D. (2015). Lymphocystis disease virus: its importance in aquaculture. Reviews in Aquaculture, 9(2), 179–193. doi:10.1111/raq.12131.
Bovo, B. & Florio, D. (2008). Viral Diseases of Cultured Marine Fish. 1st Ed. CRC Press. P. 54.
Bucke, D. (1986). A short note on a rare occurrence of Lymphocystis in Dover sole Solea solea L. Bull. Eur. Ass. Fish pathol. 6 (l), 7.
Bunkley-Williams, L., Grizzle, J.M, & Williams Jr, E.H (1996). First Report of Lymphocystis in the Family Gerreidae: Caitipa Mojarras Diapterus rhombeus from La Parguera, Puerto Rico. Journal of Aquatic Animal. Vol. 8, 2:176-179. Doi.org/10.1577/15488667(1996)008<0176:FROLIT>2.3.CO;2
Cano, I., Ferro, P., Alonso, M.C., Bergmann, S.M., Romer-Oberdorfer, A., Garcia-Rosado, E., Castro, D, & Borrego, J.J. (2006). Development of molecular techniques for detection of lymphocystis disease virus in different marine fsh species. Journal Applied Microbiology. 102, 32–40.
Cano, I., Alonso, M.C., Garcia-Rosado, E., Saint-Jean S.R., Castro, D., & Borrego, J.J. (2006). Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata L.) using an immunoblot technique. Veterinary Microbiology, 113, 137-141.
Cano, I., Lopez-Jimena, B, Garcia-Rosado, E., Ortiz-Delgado, J.B., Alonso, M.C., Borrego, J.J., Sarasquete, C., & Castro, D. (2009). Detection and persistence of lymphocystis disease virus (LCDV) in Artemia sp. Aquaculture. 291, 230–236.
Carballo, C., Castro, D., Borrego, J. J., & Manchado, M. (2017). Gene expression profiles associated with lymphocystis disease virus (LCDV) in experimentally infected Senegalese sole (Solea senegalensis). Fish & Shellfish Immunology, 66, 129–139. doi:10.1016/j.fsi.2017.04.028.
Castro, T., Mateo, E & Sierralta, V. (2018). Lymphocystic disease in the clown fish Amphiprion ocellaris (Perciformes, Pomacentridae), first report in Peru. The Biologist (Lima). Vol. 12, No.2.
Cordero, H., Cuesta, A., Meseguer, J. & Esteban, M.A. (2016). Characterization of the gilthead seabream (Sparus aurata L.) immune response under a natural lymphocystis disease virus outbreak. Journal of Fish Diseases 2016 doi:10.1111/jfd.12481.
Cheng, M.C., See, M.S., Wang, P.C., Kuo, Y.T., Ho, Y.S., Chen, S.C., & Tsai, M.A. (2022). Lymphocystis disease virus infection in clownfish Amphiprion ocellaris and Amphiprion clarkii in Taiwan. Animals 2023, 13, 153. https://doi.org/10.3390/ ani13010153.
Cherif N, Amdouni F, Maatoug K, Zaafran S (2020) Case Report: First occurrence of Lymphocystis disease virus 3 (LCDV-Sa) in Wild Marine Fish in Tunisia. Ann Mar Sci 4(1): 024-029. DOI: https://dx.doi.org/10.17352/ams.000022
Ciulli, S., Pinheiro, A. C. de A. S., Volpe, E., Moscato, M., Jung, T. S., Galeotti, M., Prosperi, S. (2015). Development and application of a real-time PCR assay for the detection and quantitation of lymphocystis disease virus. Journal of Virological Methods, 213, 164–173. doi:10.1016/j.jviromet.2014.11.01.
Cheng, M., See, M.S., Wang, P., Kuo, Y., Ho, Y., Chen, S., & Tsai, M. (2022). Lymphocystis Disease Virus Infection in Clownfish Amphiprion ocellaris and Amphiprion clarkii in Taiwan. Animals: An Open Access Journal from MDPI, 13. DOI:10.3390/ani13010153
Christmast, J.Y., & Howse, H.D. (1970). The occurrence of lymphocystis in Micropogon undulatus and Cynoscion arenarius from Mississippi Estuaries. Gulf Res. Rep., 3(1).
Chung, C. K., Kim, B.G., Jung, H. W & Jung, S. J. (2015). Identification of antigenic proteins of lymphocystis disease virus (LCDV) by MALDI-TOF mass spectrometry. J. Fish Pathol., 28(3): 133~143 http://dx.doi.org/10.7847/jfp.2015.28.3.133
Colorni, A, & Diamant, A. (1995). Short communication: splenic and cardiac lymphocystis in the red drum, Sciaenops ocellatus (L.). Journal of Fish Diseases 1995. 18, 467-471.
Darai, G., Anders, K., Koch, H.G., Delius, H., Gelderblom, H., Samalecos, C, & Flugel, R.M. (1983). Analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumours of pleuronectes. Virology 126, 466–479.
Doszpoly, A., Kaján, G.L., Puentes, R., & Perretta, A. (2020). Complete genome sequence and analysis of a novel lymphocystivirus detected in whitemouth croaker (Micropogonias furnieri): lymphocystis disease virus 4. Archives of Virology, 165, 1215 - 1218. DOI:10.1007/s00705-020-04570-1.
Fautin, D.G. & Allen, G.R. (1997). Anemone Fishes and their host sea anemones. Minneapolis, USA: Voyageur Press, 160 pp.
Genten, F., Terwinghe, E., & Danguy, A. (2009). Atlas of Fish Histology. CRC Press.
Golomazou, E., & Panagiotaki, P. (2018). Lymphocystis Disease Virus (LCDV) in Aquatic Environment. Reference Module in Earth Systems and Environmental Sciences. doi:10.1016/b978-0-12-409548-9.10968-6.
Hadfield, C. A. (2021). Viral Diseases. Clinical Guide to Fish Medicine, 407–430. doi:10.1002/9781119259886.ch24.
Hick, P., Becker, J., & Whittington, R. (2016). Iridoviruses of Fish. Aquaculture Virology,127–152. doi:10.1016/b978-0-12-801573-5.00008-5.
Hossain, M, Song J.Y, Kitamura S.I, Jung S.J, & Oh M.J. (2008). Phylogenetic analysis of lymphocystis disease virus from tropical ornamental fish species based on a major capsid protein gene. Journal of Fish Disease, 31: 473-479.
Hossain, M., Song, J.Y., Kitamura, S.I., Jung, S.J., & Oh, M.J. (2009). Phylogenetic analysis of lymphocystis disease virus from tropical ornamental fish species based on a major capsid protein gene. Journal of Fish Disease, 31, 473-479.
Hossain, M, Jung, S.J, Kim, W.S, Kim, S.R, & Oh, M.J. (2009). Comparison of lymphocystis disease virus proteins between marine and freshwater fish. Journal of Fish Pathology, 22(2): 173-177.
Howse, H.D. & Christmas, J.Y. (1970). Lymphocystis tumors: histochemical identification of hyaline substances. Trans. Amer. Microsc. Soc. 89, 276-282.
Huang, X., Huang, Y., Xu, L., Wei, S., Ouyang, Z., Feng, J & Qin, Q. (2015). Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China. Journal of Fish Diseases 2015, 38, 379–387 doi:10.1111/jfd.12244
Huizinga, H.W. & Cosgrove, G.E. (1973). Surface and visceral lymphocystis disease in a white crappie (Pomoxis annularis). Journal of Wildlife Diseases 9, 349-351.
Ignatius, B, Rathore, G, Jagdis, I, Kandasami D, & Victor, A.C. (2001). Spawning, and larval rearing technique for tropical clownfish Amphiprion sebae under captive conditions. J Aquac Tropic, 16(3): 241-249.
Iwakiri, S., Song, J., Nakayama, K., Oh, M., Ishida, M., & Kitamura, S. (2014). Host responses of Japanese flounder Paralichthys olivaceus with lymphocystis cell formation. Fish & shellfish immunology, 38 2, 406-11. DOI:10.1016/j.fsi.2014.03.028.
Kawato, S., Nozaki, R., Hirono, I., & Kondo, H. (2021). Genome sequence of lymphocystis disease virus 2 LCDV-JP_Oita_2018, Isolated from a diseased japanese flounder (Paralichthys olivaceus) in Japan. Microbiology Resource Announcements, 10. DOI:10.1128/MRA.00547-21.
Kitamura, S.I., Jung, S.J., Kim, W.S., Nishizwa, T., Yoshimizu, M, & Oh, M.J. (2006). A new genotype lymphocystis, LCDV-RF, from lymphocytes diseased rockfish. Archives of Virology, 151(3), 607-615.
Kitamura, S.I., Ko, J.Y., Lee, W.L., Kim, S.R., Song, J.Y., Kim, D.K., Jung, S.J & Oh, M.J. (2007). Seasonal prevalence of lymphocystis disease virus and aquabirnavirus in Japanese flounder, Paralichthys olivaceus and blue mussel, Mytilus galloprovincialis. Aquaculture. 266. 26-31. DOI. 10.1016/j.aquaculture.2007.02.034.
Labella, A.M., Leiva-Rebollo, R., Alejo, A., Castro, D. & Borrego, J.J. (2019). Lymphocystis disease virus (LCDV-Sa), polyomavirus 1 (SaPyV1), and papillomavirus 1 (SaPV1) in samples of Mediterranean gilthead seabream. Dis Aquat Org. Vol. 132: 151–156. https://doi.org/10.3354/dao03311
Lam, C., Khairunissa., Damayanti, L., Kurobe, T., Teh, S.J., Pfahl, H., Rapi, S., Janetski, N., & Baxa, D.V. (2020). Detection of new strain of lymphocystis disease virus (LCDV) in captive-bred clownfish Amphiprion percula in South Sulawesi, Indonesia. Aquaculture International, 28, 2121-2137. https://doi.org/10.1007/s10499-020-00581-6.
Le Deuff, R.M. & Renault, T. (1993). Lymphocystis outbreaks in farmed seabream, Sparus aurata, first report on French Mediterranean coast. Bulletin of European Association of Fish Pathology, 13, 130-133.
Liversidge, J. & Munro, A.L.S. (l978). In: Fish Pathology (ed. by R.J. Roberts), pp l28-130. Bailliere Tindall, London.
López-Bueno, A., Mavian, C.N., Labella, A.M., Castro, D., Borrego, J.J., Alcamí, A., & Alejo, A. (2016). Concurrence of Iridovirus, Polyomavirus, and a Unique Member of a New Group of Fish Papillomaviruses in Lymphocystis Disease-Affected Gilthead Sea Bream. Journal of Virology, 90, 8768 - 8779. DOI:10.1128/JVI.01369-16.
Lusiastuti, A.M. Sihananto, B.S., Wianty, C., Putri, A.W.M, & Penataseputro, T. (2022). Etioloy, Detection and control of pearl tumor disease on snakehead, Channa striata. Prosiding Seminar Nasional Ikan XI. DOI:https://doi.org/10.32491/Semnasikan-MII-2022-p.1-7
Marcogliese, D.J., Fournier, M., Lacroix, A, & Cyr, D.G. (2001). Non-specific immune response associated with infections of lymphocystis disease virus in American plaice, Hippoglossoides platessoides (Fabricius). Journal of Fish Disease. Vol. 24. 121-124. https://doi.org/10.1046/j.1365-2761.2001.00268.x
Mellergaard S, & Nielsen E. (1995). Impact of oxygen deficiency on the disease status of common dab Limanda limanda. Dis Aquat Organ. 22: 101-114.
Mosharrof H, & Myung-Joo O. (2011). Histopathology of marine and freshwater fish lymphocystis disease virus (LCDV). Sains Malaysiana 2011; 40(10): 1049-1052.
Murwantoko, Wijayanti, E., Agustatik, S., Valianti, H., Yulanda, LK., & Nur Nukmah, L.F. Detection and genotype determination of lymphocystis disease virus infecting orange clownfish, Amphiprion percula Farmed in Batam, Indonesia. Indonesian Aquaculture Journal, 17 (2), 2022, 165-172. DOI: http://dx.doi.org/10.15578/iaj.17.2.2022.165-172.
Nair, R.R., John, K.R., Krishnan, R., Gopi, A., & Safeena, M.P. (2023). PCR detection and phylogenetic analysis of the major capsid protein gene of Lymphocystis disease virus from cultured Asian seabass (Lates calcarifer Bloch, 1790) along the west coast of Kerala, India. Aquaculture International. DOI:10.1007/s10499-023-01203-7
Neave, F. (1936a). Origin of the teleost scale-pattern and the development of the teleost scale. Nature (Lond.), 137: 1034-1035.
Neave, F. 1936b. The development of the scales of Salmo. Trans. Roy. Soc. Can., Sect. V, 30: 55-72.
Neave F. (1936b) The development of the scales of Salmo.r. Trans. Sot. Can. 5, 55.-72
Nigrelli, R.F. & Ruggieci, G.D. (1965). Studies in virus diseases of fishes. Spontaneous and experimentally induced cellular hypertrophy (Lymphocystis disease) in fishes of the New York Aquarium with a report of a new cases and an annotated bibliography (1874-1965). Zoologica, 50: 83-96.
Nigrelli, R.F, & Smith, G.M. (1939). Studies in lymphocystis disease in the orange filefish, Ceratacacanthus schoepfi (Walbaum), from Sandy Hook Bay, N. J. Zoologica, 24: 255-266.
Oh, M.J., Kim, W.S., Kitamura, S.I., Lee, H., Son, B.W, & Jung, T.S. (2006). Change of pathogenecity on olive flounder, Paralichthys olivaceus by Co-Infection of Vibrio harveyi, Edwardsiella tarda, and marine birna virus. Aquaculture, 257: 156-160.
Overstreet, R.M. (1988). Aquatic pollution problems, Southeastern U.S. Coast: histopathological indicator. Aquat. Toxicol. 11, 213–239.
Paperna, I., Sabnai, I, & Colorni, A. (1982). An outbreak of lymphocystis in Sparus aurata L. In the Gulf of Aqaba, Red Sea. Journal of Fish Disease. 5, 433–437.
Pekmez, K., M. Kaplan, A.A. Çağırgan, F. Arslan, B. Kafa, & G. Kalaycı. (2022). Molecular characterization of lymphocystis disease virus detected from sea bream in Turkey. Bulletin of the European Association of Fish Pathologists 42 (2): 46–58. https://doi.org/10.48045/001c.38086.
Peters, N, & Schmid, W. (1995). Formation and disintegration of virions in lymphocystis cells of plaice Pleuronectes platessa. Dis. Aquat. Org. Vol. 21: 109-113.
Pirarat, N., Pratakpiriya, W., Jongnimitpaiboon, K., Sajjawiriyakul, K., Rodkhum, C., & Chansue, N. (2011). Lymphocystis disease in cultured false clown anemone fish (Amphiprion ocellaris). Aqua- culture, 315, 414-416.
Qin, W.Q., Gin, K.Y., Lee, L.Y., Gedaria, A.I, & Zhang, S. (2006). Development of cytometry-based method for rapid and sensitive detection of a novel marine fish iridovirus in cell culture. Journal of Virology Methods, 125, 49-54.
Ross, R.M. (1978). Territorial behaviour and ecology of the anemone fish Amphiprion melanopus on Guam. Z Tierpsychol. 46: 71- 83.
Russel, P.H. (1974). Lymphocystis in wild place Pleuronectes platessa (L.), and flounder Platichthvs fiesus (L.) in British coastal waters: a histopathological and serological study. Journal of Fish Biology 6: 771-778.
Sahoo, P.K., & Goodwin, A.E. (2012). Viruses of freshwater finfish in the Asian–Pacific Region. Indian Journal of Virology, 23, 99 - 105.
Schaperclaus, W. (1992). Fish Diseases. Volume I dan 2, A.A. Balkema, Rotterdam, p 525.
Shahi, N., Mallik, S. K., Kala, K., Joshi, N., Patiyal, R. S., Chandra, S., Singh, S & Sarma, D. (2020). Seasonal emergence of benign epidermal tumor in farm-reared adult grass carp (Ctenopharyngodon idella) caused by lymphocystis disease virus at Uttarakhand, India. Aquaculture, 735408. doi:10.1016/j.aquaculture.2020.7.
Shawky, M., Taha, E., Ahmed, B.M., Mahmoud, M.A., Abdelaziz, M., Faisal, M., & Yousif, A. (2021). Initial Evidence That Gilthead Seabream (Sparus aurata L.) Is a Host for Lymphocystis Disease Virus Genotype I. Animals: An Open Access Journal from MDPI, 11. DOI:10.3390/ani11113032.
Sheng, X.Z, & Zhan W.B. (2006). Histopathological studies on the target organs of lymphocystis disease virus of fish. Periodical Ocean University of China 2006; 36: 749-753.
Sheng, X.Z., Zhan, W.B., Xu, S.J, & Cheng, S.F. (2007). Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured. Research 6 (4), 378–382.
Sihananto, B.S., Novita, H., Wianty, C., & Lusiastuti, A.M. (2019). A Case Study of Lymphocystis Virus Disease in Farmed Giant Snakehead (Chana striata) in Mandiangin, South Kalimantan. Indonesian Aquaculture Journal, 14(2), 75-81. https://doi.org/ 10.15578/iaj.14.2.2019.75-81.
Sindermann, C.J. (1970). Principal diseases of marine fish and shellfish .52-57., Academic press. New York.
Siva, M.U., Marudhupandi, T., Haq, M.A.B, & Kumar, T.T.A. (2014). Histopathological study of lymphocystis disease virus (LCDV) in cultured false clownfish, Amphiprion ocellaris (Cuvier, 1830) and true clownfish, Amphiprion percula (Lacepede, 1802) Journal of Coastal Life Medicine. 2014; 2(4): 264-269. Doi:10.12980/JCLM.2.2014APJTD-2014-0016.
Smail, D.A. (1979). Studies on the identification and characterisation of certain fish viruses with special reference to lymphocystis and piscine erythrocytic necrosis (PEN) viruses. University of Plymouth. Theses.
Sumithra, T.G., Krupesha Sharma, S.R., Neelima, L., Dhanutha, N.R., Joshy, A., Anusree, V.N., Gayathri, S., Raghu, R., Praveen, N.D., Thomas, S., & Rajesh, K.M. (2022). Red sea bream iridovirus infection in cage farmed Asian sea bass (Lates calcarifer): Insights into the pathology, epizootiology, and genetic diversity. Aquaculture. DOI:10.1016/j.aquaculture.2021.737571.
Tidona, C.A, & Darai, G. (1997). The Complete DNA Sequence of Lymphocystis Disease Virus. Virology 230, 207–216.
Valverde, E.J., Cano, I., Labella, A.M., Borrego, J.J., & Castro, D. (2016). Application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream. BMC Veterinary Research, 12.
Valverde, E.J., Borrego, J.J., Sarasquete, M.C., Ortiz- Delgado, J.B., & Castro, D. (2017). Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata). Veterinary Research, 48, 21.
Valverde, E.J., Labella, A.M., Borrego, J.J. & Castro, D. (2019). Artemia spp., a Susceptible Host and Vector for Lymphocystis Disease Virus. Viruses: An Open Access Journal from MDPI. 11.506; doi:10.3390/v11060506.
Van Oosten J. (1957). The skin and scales. In: The physiology of fishes. Vol. l. Brown M.E. (ed.). Academic Press. New York. pp 207-244.
Vijayapoopathi, S., Ayyaru, G., Kuzhanthaivel, R., & Syed, A. (2016). Lymphocystis in Indian oil sardine, Sardinella longiceps (Valenciennes 1847). Asian Pacific Journal of Tropical Disease, 6(8), 611–614. doi:10.1016/s2222-1808(16)61095-2.
Volpe, E., Errani, F., Mandrioli, L., & Ciulli, S. (2023). Advances in Viral Aquatic Animal Disease Knowledge: The Molecular Methods’ Contribution. Biology, 12. DOI:10.3390/biology12030466
Wachirachaipaisal, N., & Limsuwan, C. (1985). Study on lymphocystis disease in red solider fish (Sarcocentrum rubrum). Conference on the living aquatic resources. Bangkok, pp. 7–8. March 1985.
Walker R. (1962). Fine Structure of lymphocystis disease virus in fish. Virology 1962; 18: 503-505.
Walker, R, & Wolf, K. (1962). Virus array in lymphocystis cells of sunfish. Am Zool, 2: 566.
Walkcr, R. (1965). Viral DNA and cyroplasmic RNA in lyrnphocystis cells of Fish in "viral Diseases of poikilothermic vertebrates” Ann. N.Y. Acad. Sci. 126: 375.
Walker, R, & Wcissenberg. (1965). Conformity of light and clcctron-microscopic studies on virus particlc distriburion in lymphocystis cells of fishes in " Viral Diseases of Poikilothermic Vertebrates” Ann. N.Y. Acad. Sci. 126:386
Walker, R, & Hill, B.J. (1980). Studies on the culture assay of infectivity and some in vitro properties of lymphocystis virus. J. Gen. Virol. 51, 385–395.
Weissenberg, R. (1965). Fifty Years of research on the lymphocystis disease of fishes (1914-1964). Ann N Y Acad Sci. 126: 362-374.
Welling, S.R. (1970). Biology of some virus diseases ol marine fish. A Symposium of the American Fishcries Society on Diseases of Fishes and Shcllfishes (ed. S.F. Snieszko). Spec. Publ. Am. Fish. Soc. (5): 296-306.
Williams Jr., E.H., Grizzle, J.M. & Bunkley-Williams, L. (1996). Lymphocystis In Indian glassfish Chanda ranga imported from Thailand to Puerto Rico. J. Aquat. Anim. Health 8, 173–175. Doi.org/10.1577/1548-8667(1996)008%3C0173:LIIGCR%3E2.3.CO;2
Woodcock, H.M. (1904). Note on a remarkable parasite of plaice and flounder. Proc. Trans. l.'Pool Biol. Soc. 18: 143
Wolf, K, (1988). Lymphocystis disease. In: Wolf K, editor. Fish viruses and fish viral diseases. Ithaca: Comstock Pub Assoc.
Wu, R.-H., Tang, X.-Q., Sheng, X.-Z., & Zhan, W.-B. (2015). Tissue distribution of the 27.8 kDa receptor and its dynamic expression in response to lymphocystis disease virus infection in Flounder (Paralichthys olivaceus). Journal of Comparative Pathology, 153(4), 324–332. doi:10.1016/j.jcpa.2015.10.176.
Xu, L., Feng, J., & Huang, Y. (2014). Identification of lymphocystis disease virus from paradise fish Macropodus opercularis (LCDV-PF). Archives of Virology, 159, 2445-2449. DOI:10.1007/s00705-014-2060-0.
Zhang, Q.Y., Xiao, F., Xie, J., Li, Z.Q, & Gui, J.F., (2004). Complete genome sequence of lymphocystis disease virus isolated from China. J. Virol. 78(13), 6982–6994.
Zhang H, Sheng X, Tang X, Xing J, Chi H and Zhan W (2023) Transcriptome analysis reveals molecular mechanisms of lymphocystis formation caused by lymphocystis disease virus infection in flounder (Paralichthys olivaceus). Front. Immunol. 14:1268851. doi: 10.3389/fimmu.2023.1268851.
Zheng, F., Liu, H., Guo, X., & Wang, B. (2016). Isolation and identification of a new isolate of lymphocystis disease virus isolated from black rockfish (Sebastes schlegelii) in China. Aquaculture, 451, 340-344. DOI:10.1016/J.AQUACULTURE.2015.09.026.
Zhong, Y., Fei, C., Tang, X., Zhan, W. & Sheng, X. (2017). A 32 kDa viral attachment protein of lymphocystis disease virus (LCDV) specifically interacts with a 27.8 kDa cellular receptor from flounder (Paralichthys olivaceus). Journal of General Virology. 98:1477–1488 DOI 10.1099/jgv.0.000805.
Zwillenberg L.D, & Wolf, K. (1962). Ultrastructure of lymphocystis virus. J Virol 1968; 2: 393-399.